مكورة معوية برازية

يجب ألا يـُخلـَط بينها وبين Enterococcus faecium.

مكورة معوية برازية
Enterococcus faecalis20023-300.jpg
التصنيف العلمي
Domain:
[[جراثيم
 الجراثيم]]
مملكة:
Eubacteria
Phylum:
Firmicutes
Class:
Coccus
Order:
Lactobacillales
Family:
Enterococcaceae
Genus:
Enterococcus
Species:
E. faecalis
Binomial name
Enterococcus faecalis
(Andrewes and Horder, 1906) Schleifer and Kilpper-Bälz, 1984

Enterococcus faecalis – formerly classified as part of the group D Streptococcus system – is a Gram-positive, commensal bacterium inhabiting the gastrointestinal tracts of humans and other mammals.[1] Like other species in the genus Enterococcus, E. faecalis is found in healthy humans, but can cause life-threatening infections, especially in the nosocomial (hospital) environment, where the naturally high levels of antibiotic resistance found in E. faecalis contribute to its pathogenicity.[1] E. faecalis has been frequently found in reinfected, root canal-treated teeth in prevalence values ranging from 30% to 90% of the cases.[2] Re-infected root canal-treated teeth are about nine times more likely to harbor E. faecalis than cases of primary infections.[3]

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الفسيولوجيا

E. faecalis is a nonmotile microbe; it ferments glucose without gas production, and does not produce a catalase reaction with hydrogen peroxide. It produces a reduction of litmus milk, but does not liquefy gelatin. It shows consistent growth throughout nutrient broth which is consistent with being a facultative anaerobe. It catabolizes a variety of energy sources, including glycerol, lactate, malate, citrate, arginine, agmatine, and many keto acids. Enterococci survive very harsh environments, including extremely alkaline pH (9.6) and salt concentrations. They resist bile salts, detergents, heavy metals, ethanol, azide, and desiccation. They can grow in the range of 10 to 45 °C and survive at temperatures of 60 °C for 30 min.[4]


الإمراض

E. faecalis is found in most healthy individuals, but can cause endocarditis and sepsis, urinary tract infections (UTIs), meningitis, and other infections in humans.[5][6] Several virulence factors are thought to contribute to E. faecalis infections. A plasmid-encoded hemolysin, called the cytolysin, is important for pathogenesis in animal models of infection, and the cytolysin in combination with high-level gentamicin resistance is associated with a five-fold increase in risk of death in human bacteremia patients.[7][8][9] A plasmid-encoded adhesin[10] called "aggregation substance" is also important for virulence in animal models of infection.[8][11]

This is a Gram stain for Enterococcus faecalis under 1000 magnification (bright field microscopy)

مقاومة مضادات الجراثيم

مقاومة الأدوية المتعددة

المقالة الرئيسية: Vancomycin-resistant Enterococcus

E. faecalis is resistant to many commonly used antimicrobial agents (aminoglycosides, aztreonam, cephalosporins (see below), clindamycin, the semisynthetic penicillins nafcillin and oxacillin, and trimethoprim-sulfamethoxazole).[بحاجة لمصدر]

Resistance to vancomycin in E. faecalis is becoming more common.[12][13] Treatment options for vancomycin-resistant E. faecalis include nitrofurantoin (in the case of uncomplicated UTIs),[14] linezolid, and daptomycin, although ampicillin is preferred if the bacteria are susceptible.[15] Quinupristin/dalfopristin can be used to treat Enterococcus faecium but not E. faecalis.[15]

In root-canal treatments, NaOCl and chlorhexidine (CHX) are used to fight E. faecalis before isolating the canal. However, recent studies determined that NaOCl or CHX showed low ability to eliminate E. faecalis.[16]

تطور مقاومة المضادات الحيوية

المقالة الرئيسية: Enterococcus § Antibacterial resistance

علاجات الأدوية المجمعة

According to one study combined drug therapy has shown some efficacy in cases of severe infections (e.g. heart valves infections) against susceptible strains of E. faecalis. An ampicillin- and vancomycin-sensitive E. faecalis (lacking high-level resistance to aminoglycosides) strains can be treated by gentamicin and ampicillin antibiotics. A less nephrotoxic combination of ampicillin and ceftriaxone (even though E. faecalis is resistant to cephalosporins, ceftriaxone is working synergistically with ampicillin) may be used alternatively for ampicillin-susceptible E. faecalis.[17]

Daptomycin or linezolid may also show efficacy in case ampicillin and vancomycin resistance.[17]

A combination of penicillin and streptomycin therapy was used in the past.[17]

عوامل البقاء والفوعة

  • Endures prolonged periods of nutritional deprivation
  • Binds to dentin and proficiently invades dentinal tubules
  • Alters host responses
  • Suppresses the action of lymphocytes
  • Possesses lytic enzymes, cytolysin, aggregation substance, pheromones, and lipoteichoic acid
  • Utilizes serum as a nutritional source
  • Resists intracanal medicaments (e.g. calcium hydroxide)
    • Maintains pH homeostasis
    • Properties of dentin lessen the effect of calcium hydroxide
  • Competes with other cells
  • Forms a biofilm[4]
  • Activates the host protease plasminogen in a fashion that increases local tissue destruction[18]

التاريخ

Prior to 1984, enterococci were members of the genus Streptococcus; thus, E. faecalis was known as Streptococcus faecalis.[19]

In 2013, a combination of cold denaturation and NMR spectroscopy was used to show detailed insights into the unfolding of the E. faecalis homodimeric repressor protein CylR2.[20]

تركيب الجينوم

The E. faecalis genome consists of 3.22 million base pairs with 3,113 protein-coding genes.[21]

الرنا الصغير

Bacterial small RNAs play important roles in many cellular processes; 11 small RNAs have been experimentally characterised in E. faecalis V583 and detected in various growth phases.[22] Five of them have been shown to be involved in stress response and virulence.[23]

A genome-wide sRNA study suggested that some sRNAs are linked to the antibiotic resistance and stress response in another Enteroccocus: E. faecium.[24]

انظر أيضاً

المصادر

  1. ^ أ ب Ryan KJ, Ray CG, eds. (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. pp. 294––5. ISBN 0-8385-8529-9.
  2. ^ Molander, A.; Reit, C.; Dahlén, G.; Kvist, T. (1998). "Microbiological docks of root-filled teeth with apical periodontitis". International Endodontic Journal. 31 (1): 1–7. doi:10.1046/j.1365-2591.1998.t01-1-00111.x. PMID 9823122.
  3. ^ Rocas, I.; Siqueira, J.; Santos, K. (2004). "Association of Enterococcus faecalis With Different Forms of Periradicular Diseases". Journal of Endodontics. 30 (5): 315–320. doi:10.1097/00004770-200405000-00004. PMID 15107642.
  4. ^ أ ب Stuart, C. H.; Schwartz, S. A.; Beeson, T. J.; Owatz, C. B. (2006). "Enterococcus faecalis: Its role in root canal treatment failure and current concepts in retreatment". Journal of Endodontics. 32 (2): 93–8. doi:10.1016/j.joen.2005.10.049. PMID 16427453.
  5. ^ Murray, BE. (Jan 1990). "The life and times of the Enterococcus". Clin Microbiol Rev. 3 (1): 46–65. doi:10.1128/cmr.3.1.46. PMC 358140. PMID 2404568.
  6. ^ Hidron AI, Edwards JR, Patel J (November 2008). "NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007". Infect Control Hosp Epidemiol. 29 (11): 996–1011. doi:10.1086/591861. PMID 18947320. {{cite journal}}: Unknown parameter |displayauthors= ignored (|display-authors= suggested) (help)
  7. ^ Huycke, MM.; Spiegel, CA.; Gilmore, MS. (Aug 1991). "Bacteremia caused by hemolytic, high-level gentamicin-resistant Enterococcus faecalis". Antimicrob Agents Chemother. 35 (8): 1626–34. doi:10.1128/aac.35.8.1626. PMC 245231. PMID 1929336.
  8. ^ أ ب Chow, JW.; Thal, LA.; Perri, MB.; Vazquez, JA.; Donabedian, SM.; Clewell, DB.; Zervos, MJ. (Nov 1993). "Plasmid-associated hemolysin and aggregation substance production contribute to virulence in experimental enterococcal endocarditis". Antimicrob Agents Chemother. 37 (11): 2474–7. doi:10.1128/aac.37.11.2474. PMC 192412. PMID 8285637.
  9. ^ Ike, Y.; Hashimoto, H.; Clewell, DB. (Aug 1984). "Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice". Infect Immun. 45 (2): 528–30. doi:10.1128/IAI.45.2.528-530.1984. PMC 263283. PMID 6086531.
  10. ^ Wirth, R.; Schramm, U.; Marre, R.; Kreft, B. (January 1992). "Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells". Infection and Immunity. 60 (1): 25–30. doi:10.1128/IAI.60.1.25-30.1992. PMID 1729187.
  11. ^ Hirt, H.; Schlievert, PM.; Dunny, GM. (Feb 2002). "In vivo induction of virulence and antibiotic resistance transfer in Enterococcus faecalis mediated by the sex pheromone-sensing system of pCF10". Infect Immun. 70 (2): 716–23. doi:10.1128/iai.70.2.716-723.2002. PMC 127697. PMID 11796604.
  12. ^ Amyes SG (May 2007). "Enterococci and streptococci". Int. J. Antimicrob. Agents. 29 Suppl 3: S43–52. doi:10.1016/S0924-8579(07)72177-5. PMID 17659211.
  13. ^ Courvalin P (January 2006). "Vancomycin resistance in Gram-positive cocci". Clin. Infect. Dis. 42 Suppl 1: S25–34. doi:10.1086/491711. PMID 16323116.
  14. ^ Zhanel GG, Hoban DJ, Karlowsky JA (January 2001). "Nitrofurantoin is active against vancomycin-resistant enterococci". Antimicrob. Agents Chemother. 45 (1): 324–6. doi:10.1128/AAC.45.1.324-326.2001. PMC 90284. PMID 11120989.
  15. ^ أ ب Arias, CA.; Contreras, GA.; Murray, BE. (Jun 2010). "Management of multidrug-resistant enterococcal infections". Clin Microbiol Infect. 16 (6): 555–62. doi:10.1111/j.1469-0691.2010.03214.x. PMC 3686902. PMID 20569266.
  16. ^ Estrela, C; Silva, J. A.; De Alencar, A. H.; Leles, C. R.; Decurcio, D. A. (2008). "Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis--a systematic review". Journal of Applied Oral Science. 16 (6): 364–8. doi:10.1590/s1678-77572008000600002. PMC 4327704. PMID 19082392.
  17. ^ أ ب ت Dubin K, Pamer EG (November 2014). "Enterococci and Their Interactions with the Intestinal Microbiome". Microbiology Spectrum. 5 (6). doi:10.1128/microbiolspec.BAD-0014-2016. PMC 5691600. PMID 29125098. Severe cases of enterococcal infections, such as infections of heart valves, has relied on combination drug therapy
  18. ^ Jacobson, R. A., Wienholts, K., Williamson, A. J., Gaines, S., Hyoju, S., Van Goor, H., ... & Alverdy, J. C. (2019). Enterococcus faecalis exploits the human fibrinolytic system to drive excess collagenolysis: implications in gut healing and identification of druggable targets. American Journal of Physiology. Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.00236.2019
  19. ^ Schleifer KH, Kilpper-Balz R (1984). "Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov". Int. J. Syst. Bacteriol. 34: 31–34. doi:10.1099/00207713-34-1-31.
  20. ^ Jaremko M, Jaremko L, Kim HY, Cho MK, Schwieters CD, Giller K, Becker S, Zweckstetter M (2013). "Cold denaturation of a protein dimer monitored at atomicresolution". Nat. Chem. Biol. 9 (4): 264–270. doi:10.1038/nchembio.1181. PMC 5521822. PMID 23396077.
  21. ^ Paulsen, IT.; Banerjei, L.; Myers, GS.; Nelson, KE.; Seshadri, R.; Read, TD.; Fouts, DE.; Eisen, JA.; et al. (Mar 2003). "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis". Science. 299 (5615): 2071–4. Bibcode:2003Sci...299.2071P. doi:10.1126/science.1080613. PMID 12663927.
  22. ^ Shioya, Kouki; Michaux, Charlotte; Kuenne, Carsten; Hain, Torsten; Verneuil, Nicolas; Budin-Verneuil, Aurélie; Hartsch, Thomas; Hartke, Axel; Giard, Jean-Christophe (2011-01-01). "Genome-wide identification of small RNAs in the opportunistic pathogen Enterococcus faecalis V583". PLOS ONE. 6 (9): e23948. Bibcode:2011PLoSO...623948S. doi:10.1371/journal.pone.0023948. ISSN 1932-6203. PMC 3166299. PMID 21912655.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  23. ^ Michaux, Charlotte; Hartke, Axel; Martini, Cecilia; Reiss, Swantje; Albrecht, Dirk; Budin-Verneuil, Aurélie; Sanguinetti, Maurizio; Engelmann, Susanne; Hain, Torsten (2014-09-01). "Involvement of Enterococcus faecalis Small RNAs in Stress Response and Virulence". Infection and Immunity (in الإنجليزية). 82 (9): 3599–3611. doi:10.1128/IAI.01900-14. ISSN 0019-9567. PMC 4187846. PMID 24914223.
  24. ^ Sinel, Clara; Augagneur, Yoann; Sassi, Mohamed; Bronsard, Julie; Cacaci, Margherita; Guérin, François; Sanguinetti, Maurizio; Meignen, Pierrick; Cattoir, Vincent (2017-09-11). "Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance". Scientific Reports. 7 (1): 11067. Bibcode:2017NatSR...711067S. doi:10.1038/s41598-017-11265-2. PMC 5593968. PMID 28894187.


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